Research Article

Epidemiological studies and antifungal sensitivity pattern of clinical isolates of dermatophytes from localized cases of canine dermatophytosis

  • Views: 92
  • Pages: 46-51
Authors:
*Corresponding Author Email:  manjukm@kvasu.ac.in

Received -  08.05.2024, Accepted -  07.08.2024, Published -  20.08.2024

Citation:  Mathew MK, Madhavan Unny N, A. George A, Ajithkumar S, Ramnath V and Suja Rani S, 2024. Epidemiological studies and antifungal sensitivity pattern of clinical isolates of dermatophytes from localized cases of canine dermatophytosis. Indian J Anim Health, 64(1): 46-51, doi: https://doi.org/10.36062/ijah.2024.06624

 Abstract

Dermatophytosis is one of the most common skin diseases in dogs caused by keratinophilic fungi of the family Arthrodermataceae. Dermatophytes of veterinary importance belong to genus Microsporum, Trichophyton and Epidermophyton. Lesser treatment options and frequent use of non-prescription antifungal drugs resulted in development of resistance to the commonly used drugs. The research was conducted to study the epidemiology of cases of localized canine dermatophytosis and to assess the antifungal sensitivity pattern of the isolated organisms. Animals tentatively diagnosed with dermatophytosis based on the presence of endothrix or ectothrix on trichogram were selected for the study. Occurrence rate of dermatophytosis was 17.66% during the study period. The dermatophytes isolated in the current study were Microsporum canis, Trichophyton mentagrophytes and Microsporum gypseum. The sensitivity pattern of the dermatophytes varied depending on the species isolated. Majority of the M. canis isolates were mainly sensitive to nystatin, M. gypseum to amphotericin B and T. mentagrophytes to itraconazole, whereas resistance was shown by all three isolates against fluconazole. Statistically significant difference was noticed in the culture results obtained on two different fungal specific medias viz, dermatophyte test medium and sabouraud dextrose agar.


Reference

Colombo S, Cornegliani L, Beccati M and Albanese F, 2010. Comparison of two sampling methods for microscopic examination of hair shafts in feline and canine dermatophytosis. Veterinaria, 24(3): 27-33

Debnath C, Mitra T, Kumar A and Samanta I, 2016. Detection of dermatophytes in healthy companion dogs and cats in eastern India. Iran J Vet Res, 17(1): 20-24, doi: 10.22099/IJVR.2016.3598

Dogra S, Shaw D and Rudramurthy SM, 2019. Antifungal drug susceptibility testing of dermatophytes: laboratory findings to clinical implications. Indian Dermatol Online J, 10: 225-233, doi: 10.4103/idoj.IDOJ_146_19

Kaufmann R, Blum SE, Elad D and Zur G, 2016. Comparison between point-of-care dermatophyte test medium and mycology laboratory culture for diagnosis of dermatophytosis in dogs and cats. Vet Dermatol, 27(4): 284-290, doi: 10.1111/vde.12322

Klatte JL, van der Beek N and Kemperman PM, 2015. 100 years of Wood's lamp revised. J Eur Acad Dermatol Venereol, 29(5): 842-847, doi: 10.1111/jdv.12860

Malleswari T, Davis JK, Vinodkumar K, Vijayakumar K and Menon V, 2022. Occurrence of dermatophytosis in dogs from Thrissur, Kerala. J Vet Anim Sci, 53 (2): 322-327, doi: 10.51966/jvas.2022.53.2.322-327

Marchegiani A, Fruganti A, Spaterna A, Dalle Vedove E, Bachetti B et al., 2020. Impact of nutritional supplementation on canine dermatological disorders. Vet Sci, 7(2): 38-52, doi: 10.3390/vetsci7020038

Moriello KA and Newbury S, 2006. Recommendations for the management and treatment of dermatophytosis in animal shelters. Vet Clin North Am Small Anim Pract, 36(1): 89-114, doi: 10.1016/j.cvsm.2005.09.006

Moriello KA, Coyner K, Paterson S and Mignon B, 2017. Diagnosis and treatment of dermatophytosis in dogs and cats. Clinical consensus guidelines of the World Association for Veterinary Dermatology. Vet Dermatol, 28: 266-303, doi: 10.1111/vde.12440

Moskaluk AE and VandeWoude S, 2022. Current topics in dermatophyte classification and clinical diagnosis. Pathogens, 11(9): 957, doi: 10.3390/pathogens11090957

Salkin IF, Padhye AA and Kemma ME, 1997. A new medium for the presumptive, identification of dermatophytes. J Clin Microbiol, 35: 2660-2662, doi: 10.1128/jcm.35.10.2660-2662.1997

Scherer WP and Kinmon K, 2000. Dermatophyte test medium culture versus mycology laboratory analysis for suspected onychomycosis. A study of 100 cases in a geriatric population. J Am Podiatr Med Assoc, 90: 450-459, doi: 10.7547/87507315-90-9-450

Silver H, 2011. Dermatophytosis in cats and dogs. The Vet Nurse, 2(6): 310-316, doi: 10.12968/VETN.2011.2.6.310

Zineldar HA, Abouzeid NZ, Eisa MI, Bennour EM and Neshwy WME, 2023. Prevalence, clinical presentation, and therapeutic outcome of ectoparasitic infestations in dogs in Egypt. Open Vet J, 13(12): 1631-1644, doi: 10.5455/OVJ.2023.v13.i12.13